Agarose Gel Electrophoresis Lab Report

When the gel is cooled, the comb is removed, leaving little slots which will be used to hold DNA samples.A special characteristic of the cooled agarose mixture (called a gel matrix) stems from the fact that it is created with salt water.Gel electrophoresis is a laboratory procedure used to separate biological molecules with an electrical current.

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This mixture of agarose and buffer is heated until the two substances melt together, then poured into a forming mold.

A device called a comb is then placed at one end of the mold before the gel cools.

Gel electrophoresis is a method used in laboratories to measure and sort strands of DNA.

It is necessary because DNA under normal conditions is too small to manipulate, even when viewed using most microscopes.

The gel electrophoresis lab uses a relatively straightforward procedure, and the same basic technique can be used to separate individual proteins, as well.

To begin the gel electrophoresis procedure, you first must create the gel.

Salt water solution is poured into the bottom of the electrophoresis chamber, and the gel matrix is submerged slightly within this solution.

The salt water serves two purposes: aiding the flow of electricity and keeping the gel matrix moist.

This is a small rectangular box, wired with a positive and negative electrical connection at either end.

Chambers are typically shallow, small enough to fit on a tabletop, and built from clear materials like Plexiglas.


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