As far as we know, there is no study on the relationship between p H, power generation and temperature during the start-up of microbial fuel cells.
Zhang’s research shows that the single-chamber MFC’s power generation performance is strongly influenced by temperature, and the operation of a single-chamber MFC should be controlled primarily by temperature .  assessed the performance of air cathode, single-chambered, Tubular Microbial Fuel Cells (TMFCs) provided with Nafion membrane, according to different operating conditions.  studied the application of heme-c containing enzymes in biofuel cell design.
As the principal factor affecting the power generation of SMFC, no one has studied the comprehensive relationship between them and electricity generation.
The cathode operating conditions play an important part in the overall performance of the MFC .
Cathodic p H microenvironment is one of the crucial factors affecting the metabolic activity of the substrate, affecting the electron and H reaction mechanism.